151 Identification of Burkholderia cepacia complex using MALDI-TOF mass spectrometry
نویسندگان
چکیده
منابع مشابه
Routine identification of Nocardia species by MALDI-TOF mass spectrometry.
We here show adequate species identification for bacterial isolates of the genus Nocardia spp. through VITEK mass spectrometry. Application of a specific sample preparation method in combination with a robust matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) database leads to 94% accurate identification to the species level on a set of 164 isolates. The...
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Leptospirosis is a worldwide deadly zoonotic disease. Accurate identification of the causative Leptospira spp. spirochetes ascertains the pathogenic status of the isolates, identifies potential source of infection and recognises outbreaks. Species identification is currently based on technically demanding, time and resources consuming serological and molecular methods. Matrix-assisted laser des...
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24 A method for rapid species identification of ticks may help clinicians predict the disease outcomes 25 of patients with tick bites and may inform the decision as to whether to administer post-exposure 26 prophylactic antibiotic treatment. We aimed to establish a MALDI-TOF MS spectra database based 27 on the analysis of the legs of 6 tick vectors: Amblyomma variegatum, Rhipicephalus sanguineu...
متن کاملIdentification of fungal microorganisms by MALDI-TOF mass spectrometry.
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has emerged as a reliable tool for fast identification and classification of microorganisms. In this regard, it represents a strong challenge to microscopic and molecular biology methods. Nowadays, commercial MALDI systems are accessible for biological research work as well as for diagnostic applications...
متن کاملIdentification of disulfide-containing chemical cross-links in proteins using MALDI-TOF/TOF-mass spectrometry.
Cross-linking can be used to identify spatial relationships between amino acids in proteins or protein complexes. A rapid and sensitive method for identifying the site of protein cross-linking using dithiobis(sulfosuccinimidyl propionate) (DTSSP) is presented and illustrated with experiments using murine cortactin, actin and acyl-CoA thioesterase. A characteristic 66 Da doublet, which arises fr...
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ژورنال
عنوان ژورنال: Journal of Cystic Fibrosis
سال: 2006
ISSN: 1569-1993
DOI: 10.1016/s1569-1993(06)80135-9